Characterization of caveolin-rich membrane domains isolated from an endothelial-rich source: implications for human disease.
نویسندگان
چکیده
منابع مشابه
Characterization of caveolin-rich membrane domains isolated from an endothelial-rich source: implications for human disease
Caveolae are 50-100-nm membrane microdomains that represent a subcompartment of the plasma membrane. Previous morphological studies have implicated caveolae in (a) the transcytosis of macromolecules (including LDL and modified LDLs) across capillary endothelial cells, (b) the uptake of small molecules via a process termed potocytosis involving GPI-linked receptor molecules and an unknown anion ...
متن کاملCholesterol- and caveolin-rich membrane domains are essential for phospholipase A2-dependent EDHF formation.
OBJECTIVE Cholesterol-rich membrane domains, which contain the scaffold protein caveolin-1 (Cav-1) (caveolae), represent an important structural element involved in endothelial signal transduction. The present study was designed to investigate the role of these signaling platforms in the generation of endothelial-derived hyperpolarizing factor (EDHF). METHODS Caveolae were disrupted by choles...
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This study demonstrates that caveolae, omega-shaped membrane invaginations, are involved in cardiac sodium channel regulation by a mechanism involving the alpha subunit of the stimulatory heterotrimeric G-protein, Galpha(s), via stimulation of the cell surface beta-adrenergic receptor. Stimulation of beta-adrenergic receptors with 10 micromol/L isoproterenol in the presence of a protein kinase ...
متن کاملCaveolin scaffolding region and cholesterol-rich domains in membranes.
A protein that constitutes a good marker for a type of cholesterol-rich domain in biological membranes is caveolin. A segment of this protein has a sequence that corresponds to a cholesterol recognition/interaction amino acid consensus (CRAC) motif; this motif has been suggested to cause the incorporation of proteins into cholesterol-rich domains. We have studied the interaction of two peptides...
متن کاملSterol-rich plasma membrane domains in fungi.
Concepts regarding the eukaryotic plasma membrane have been evolving in light of growing evidence that it is segregated into distinct lateral domains known as lipid rafts. These steroland sphingolipid-rich raft domains are thought to play important roles in dynamic processes, including protein sorting, cell polarity, and signal transduction. Because of this, it has been very intriguing that ste...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1994
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.126.1.111